Genetic dissection of the pathways and phenotypes using reverse-genetics approach can be facilitated by systematic gene prioritization.
Recently, effectiveness of network-assisted gene prioritization for complex traits has been demonstrated in many organisms.
Hence we constructed a fly network prioritization server, FlyNet, which includes a network of 13,119 fly genes (~95% of the coding genes)
and 779,484 links
. FlyNet has several distinctive features that enable more versatile hypothesis generation for Drosophila-based studes
- FlyNet can prioritize both genes (gene prioritization) and functional annotations (function prioritization).
- FlyNet can use two complementary network prioritization algorithms, direct neighborhood and network diffusion.
- FlyNet can utilize a spatiotemporal-specific network (STN) as an additional prioritization strategy for traits associated with a specific development stage or tissue.
- FlyNet can prioritize Drosophila genes not only for Drosophila traits (fly prioritizer) but also for human disease (human prioritizer).